Use methods and operation steps of fermentation tank

1）、Correction pH electrode and dissolved oxygen electrode

2）、Sterilization of tank. According to needs，making the culture medium into the tank. After sealing tanks as required, put tank in large sterilization pot to sterilize (115 ℃, 30 minutes).

3）、After cooling tank, putting its on the fermentation stage and installing intactly. Open the cooling water and the air pump power, connect air duct to ventilate and make airinflow properly by adjusting airinflow button; Open fermentation tank power, set the temperature, pH, stirring speed and so on.. Start up and turn 30 min Under the condition of 640 r/min, and setting dissolved oxygen electrodes are 100.

4）、After the temperature is stable and all the parameters are correct, inoculate seeds that were cultivated, start to time the fermentation time and record all kinds of parameters.

5）、After the fermentation, clean tank and electrode, and insert electrodes into the triangle bottle with 4M KCL to reserve.